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Tunable, 2D separation of macromolecules and particles

The , in conjunction with an Eclipse® DualTec® or Eclipse AF4 asymmetrical-flow field-flow fractionation (AF4) system, separates macromolecules and particles from 1 nm to 10,000 nm by size charge.

2D separation using EAF4 and the Mobility provide two unique benefits beyond standard AF4:

The Mobility is invaluable for studying , , , of proteins and sub-micron particles, and a host of other macromolecules and colloids.

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A new dimension in FFF

Standard field-flow fractionation using AF4 is a versatile, size-based separation technique that can be tuned to adjust resolution and range. With no packed stationary phase, AF4 is free of shear, and adverse surface interactions are minimal, making it an ideal technology for complex and difficult analytical fractionation tasks.

The Mobility adds the ability to modulate separation by charge. Depending on the particle’s charge and polarity, its motion under the electric field combines with AF4 cross flow and parabolic channel flow to produce later or earlier elution. Hence analytes with the same size but different charge leave the EAF4 channel and reach the detectors at different times. Each fraction is characterized separately by the detectors.

Combining electrical fractionation with AF4 in a single separation channel in EAF4, the Mobility is the most powerful and versatile separation technology available for macromolecules and nanoparticles from 1 nm to 10,000 nm.

What's inside?

These are used with a standard Eclipse FFF controller and HPLC components from Agilent, Shimadzu or Thermo. Temperature control of the EAF4 channel is provided by a Thermos[Pro] chamber.

The electrodes, placed above and below the AF4 channel, create a programmable electric field but are never actually touched by sample. These platinum-coated steel electrodes are compatible with a large range of salt concentrations: they do not corrode, and the pressure present in the channel prevents bubble formation under electrolysis.

Rounding out the Mobility package, the VOYAGER CDS® chromatography data software and SCOUT DPS® data processing and simulation software perform optimization, control and analysis of EAF4 methods, to maximize size and zeta potential characterization.

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Learn more about EAF4 and how it determines zeta potential in: C. Johann, S. Elsenberg, H. Schuch, U. Rosch., "Instrument and Method to Determine the Electrophoretic Mobility of Nanoparticles and Proteins by Combining Electrical and Flow Field-Flow Fractionation," (8) , 4292-4298 (2015) [ DOI: 10.1021%2Fac504712n .

EAF4 is combined with one or more downstream, online detectors for particle characterization:

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The last two results clearly show that \cov(X, Y) and \cor(X, Y) measure the linear association between X and Y . The equivalent inequalities (a) and (b) above are referred to as the correlation inequality . They are also versions of the Cauchy-Schwarz inequality , named for and

Recall from our previous discussion of variance that the best constant predictor of Y , in the sense of minimizing mean square error, is \E(Y) and the minimum value of the mean square error for this predictor is \var(Y) . Thus, the difference between the variance of Y and the mean square error above for L(Y \mid X) is the reduction in the variance of Y when the linear term in X is added to the predictor: \var(Y) - \E\left(\left[Y - L(Y \mid X)\right]^2\right) = \var(Y) \, \cor^2(X, Y) Thus \cor^2(X, Y) is the proportion of reduction in \var(Y) when X is included as a predictor variable. This quantity is called the (distribution) coefficient of determination . Now let

The function x \mapsto L(Y \mid X = x) is known as the distribution regression function for Y given X , and its graph is known as the distribution regression line . Note that the regression line passes through \left(\E(X), \E(Y)\right) , the center of the joint distribution.

However, the choice of predictor variable and response variable is crucial.

The regression line for Y given X and the regression line for X given Y are not the same line, except in the trivial case where the variables are perfectly correlated. However, the coefficient of determination is the same, regardless of which variable is the predictor and which is the response.

The two regression lines are \begin{align} y - \E(Y) = \frac{\cov(X, Y)}{\var(X)}\left[x - \E(X)\right] \\ x - \E(X) = \frac{\cov(X, Y)}{\var(Y)}\left[y - \E(Y)\right] \end{align} The two lines are the same if and only if \cov^2(X, Y) = \var(X) \var(Y) . But this is equivalent to \cor^2(X, Y) = 1 .

Suppose that A and B are events with 0 \lt \P(A) \lt 1 and 0 \lt \P(B) \lt 1 . Then

The concept of best linear predictor is more powerful than might first appear, because it can be applied to transformations of the variables. Specifically, suppose that X and Y are random variables for our experiment, taking values in general spaces S and T , respectively. Suppose also that g and h are real-valued functions defined on S and T , respectively. We can find L\left[h(Y) \mid g(X)\right] , the linear function of g(X) that is closest to h(Y) in the mean square sense. The results of this subsection apply, of course, with g(X) replacing X and h(Y) replacing Y . Of course, we must be able to compute the appropriate means, variances, and covariances.

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Pleasant View: 2700 N. Hwy 89 Ogden:2393 S. Wall Ave. Roy:4155 S. Sandridge Dr.

Clearfield: 1250 S. State St. Layton:150 S. Main St. Farmington: 450 N. 850 W. Woods Cross: 750 S. 800 W.

North Temple:500 W. North Temple Salt Lake Central: 250 S. 600 W. Murray Central:140 W. Vine St South Jordan:10351 South Jordan Gateway Draper:12997 S. FrontRunner Blvd.

Salt Lake

Lehi:3101 N. Ashton Blvd. American Fork: 782 W. 200 S. Orem Central:1350 W. 900 S. Provo Central:690 S. University Ave.

Draper Town Center: 1131 E Pioneer Road Kimballs Lane: 11796 South 700 E


Crescent View: 11400 South 361 E Sandy Civic: Sego Lily Drive 115 East Sandy Expo: 9375 S 150 E Historic Sandy: 9000 South 165 East

Midvale Center: 7720 South 95 West Midvale Fort Union: 7250 South 180 West


Fashion Place West: Winchester St. 222 West Murray Central: Vine St. Cottonwood St. Murray North: Fireclay Ave 71 West


Meadowbrook: 3900 South 188 West Millcreek: 3300 South 210 West


Central Pointe: 2100 South 221 West

South Salt Lake

Ballpark: 1300 South 180 West 900 S: 900 S 200 West Court House: 450 S Main Street Gallivan Plaza: 275 S Main Street City Center: 100 S Main Street Temple Square: 155 W South Temple Arena: 350 W South Temple Planetarium: 150 S 400 West Old Greek Town: 550 W 200 S Salt Lake Cental: 325 S 600 W

Daybreak Parkway: 11405 S. Grandville Ave. South Jordan Parkway: 10605 S. Grandville Ave.

South Jordan

n 5600 W. Old Bingham Hwy: 5651 W. Old Bingham Hwy 4800 W. Old Bingham Hwy: 4773 W. Old Bingham Hwy Jordan Valley: 3400 W. 8600 S. 2700 W. Sugar Factory Rd.: 8351 S. 2700 W. West Jordan City Center: 8021 S. Redwood Road Historic Gardner: 1127 W. 7800 S.

West Jorda

Bingham Junction: 7387 S. Bingham Junction Blvd.

Central Pointe: 2100 South 221 West Ballpark: 1300 South 180 West 900 S: 900 S 200 West Court House: 450 S Main Street Library: 225 E 400 S Trolley: 625 E 400 S 900 East: 875 E 400 S Stadium: 1349 E 500 S South Campus: 1790 E South Campus Drive Fort Douglas: 200 South Mario Capecchi Drive Medical Center: 10 North Mario Capecchi Drive

West Valley Central: 2750 West 3590 South Decker Lake: 3070 South 2200 West Redwood Junction: 1740 West Research Wy River Trail: 2340 South 1070 West

West Valley

Ballpark: 1300 South 180 West 900 South: 900 S 200 West Courthouse: 450 S Main Street Gallivan Plaza: 275 S Main Street City Center: 100 S Main Street Temple Square: 155 W South Temple Arena Station: 350 W South Temple North Temple Bridge/Guadalupe: 500 W. North Temple Jackson/Euclid: 820 W. North Temple Fairpark: 1150 W. North Temple Power: 1500 W. North Temple 1940 West North Temple: 1940 W. North Temple Airport: 650 N. 3700 W.

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About Jami Balmet

Jami is passionate about Biblical Homemaking and striving to be a woman of God. Jami and her loving husband Jason, have two sets of twin boys and a baby girl on the way. She knows what it’s like to be burnt-out, overwhelmed, stressed and in the trenches of homemaking and motherhood. As a result, she seeks to point herself, her family, and her blog back to Christ all while finding joy, peace, and purpose in within her home for God’s Glory. She blogs about this passion at Young Wife's Guide and through her FREE eCourse: Small towelChildrens shoes USB charging emitting light boys shoes girls shoes luminous LED lighted sports shoes big boy shoes style c5 1AMEYADMqM
- Essential Habits for Christian Homemakers.


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  1. Timely article. I have been thinking a lot recently how homemaking is a lost art and how I married without a clue of how to be a wife and a homemaker. I can’t blame it entirely on my parents as I really had no interest in the first place in learning. In fact, I think it’s taken me 3 years of marriage to realize its even something I need to bother learning in the first place. I have a lot of work and a huge learning curve ahead of me.

  2. Breanna says:

    love the article! i think as home makers we have to realize that we will always be learning new things, especially as the kids grow and change their needs. after 11 years of marriage i am still learning all the time that i need to rework my schedule or my recipe book, but that is the best part of being at home! i feel so blessed to have this job and just hope and pray more women can realize how important their jobs are at home! thanks for the daily reminders of how amazing life as a home maker is! God bless!

  3. This is encouraging… and convicting! I haven’t spent the time studying and learning that I want to, on becoming a better homemaker. I’m learning SLOWLY, but I’m hoping that this summer I have more time to devote to increasing and strengthening my homemaking skills!

    Thank you especially for that last paragraph. I struggle with comparing myself with women who are better cooks, better housekeepers, better organized, better wives… but then, they’ve ALL had more years to hone their skills! As a young wife, I’m doing well for my age and experience level. I have a lot of years ahead of me, and therefore no reason to be discouraged about what I don’t know yet.

    Thank you, dear!

    • Thanks Jaimie!! When I was in school, Summer was my homemaking time!! And I did little things like making one new recipe a week on Friday’s nights during the school year. If you do those things consistantly, slowly, you can grow in your homemaking!!

      And I know! I do the same thing!! It’s so easy to compare ourselves to others isn’t it? And someone’s life may seem great on the outside {or online} but in reality things are falling apart!

      Thanks for the comment Jaimie! Love you!

  4. Alyssa says:

    This is a great idea. I have NO homemaking skills, I can’t even cook, but making a list is a great idea. How simple, yet I never thought of it! Thanks for sharing

  5. I have been feeling really overwhelmed with the lack of knowledge I have in the art of Homemaking and this really helped. I am sitting here writing my list now of all I want to learn, what a great idea! Thanks so much.

  6. Kate says:

    Thanks for this article! I have been enjoying reading lots of articles on your blog this week! I too am a young bride, my husband and I got married last November at just 21 and 23. While we both work outside of our little home right now, we are hoping to get pregnant with our first child soon and I plan to transition to staying at home full time.

    I realized during our engagement period how important it is to learn the art of homemaking in addition to formal schooling. I grew up with a mostly stay at home single mom so I learned basic cooking, cleaning, and home maintenance but I realized that having your own home (even if it is a little one bedroom apartment!) is so different. Since that realization I have been really trying to improve in these areas. I have a feeling that your blog will be of much help as I learn and grow!

    Keep up the good work! It’s always encouraging to find other young, Christian, wives/mommas around!

  7. Tess says:

    Timely article! I started a Pintrest account a while back specifically for keeping track of homemaking tips. Thanks so much for your tips! Will you be posting more in the future?

  8. How To Become A Better Homemaker—Make A List! | Headed Home says:

    […] recently read a post on Young Wife’s Guideabout growing as a homemaker, and I was inspired by one of her suggestions: Make a […]

  9. Everything you need to create a healthy home! {Plus Kindle Fire Giveaway} | Homemaking From Scratch says:

    […] Tomorrow I am excited to be sharing my personal goals in my homemaking and hopefully encourage you to develop a list of goals for yourself (if you don’t already have one).Being a homemaker means so much more than just staying home, homemaking is a career and an art! […]

  10. Tabi J says:

    At first, I thought that I will ended up being a married career woman, but I feel that the Lord wants me to be a homemaker and only focus getting employment in order to get out of debt, not to support my family.

  11. Alison Ray says:

    Wonderful!!!! Wow, it’s nice to meet another woman who is after the same goal! I just turned 24, and have been married for only 5 years. I am so looking forward to learning more about how to manage my home and be the best wife ever. This article is encouraging, because I have JUST this week conquered my very first curtains. Anyway, I am rambling because I live in a Christian group home with 25 men and no girls to talk home-making with. I guess I have a big, big home to manage. Thanks for the encouragement!!

  12. Samantha says:

    This is a great article and we all should strive to be a excellent homemaker and the Proverbs 31 women. A quote from Stacy Make Cents–I need my heart to be with my family 100%. I feel like that’s my calling as a wife and a mother to small children. I was trying as hard as I could to be the Proverbs 31 woman and do it all…until it was pointed out to me by a loving friend that it wasn’t possible for me to do that – and that in fact, the Proverbs 31 woman had children old enough to rise and call her “blessed.” Annie (my oldest at 4 years) arises and says “Mama, what’s for breakfast?”

    As a mother with young children This was a good reminder to me that I cant do it all! We all have to set our priorities to fit the stage of life we are in.

  13. Rebecca says:

    This is a great article on a great topic! It’s so nice to find a kindred spirit. My family and friends are confused by my homemaker status. They all ask a variation of “Why can’t you just get a job?”. I reply that I have a job- I keep my home. And my husband and I have a wonderful, peaceful, abundant life without being wealthy because we provide for each other, and the Lord provides for us.

    Thank you so much for this website! I’m truly inspired.

  14. Struggling Homemaker says:

    Thank you for this article. I am light years behind everyone here probably. I was raised in a dysfunctional home, ended up married and pregnant at 19, divorced at 24, remarried at 29 and raising four kids in a blended family. My sister passed away last year and I don’t have family in the area. I do have good friends and a great church! I recently rededicated my life to Christ. I am so completely overwhelmed when it comes to homemaking and my initial reaction is to run away….get a full time job and pay someone to clean, nanny, etc., because it is so hard for me to figure out! My house is messy…clean but cluttered…and I always feel way behind. I am really trying to find somewhere to start but being home is a very hard job, especially when you didn’t ease into it and have the husband and kids and combined homes right from the start. I was inspired by this post and I will keep reading. Thank you.

  15. One challenge that so many face in homemaking these days is that society expects it to be a side gig. Rather than devote one’s life to managing the home, preparing meals, and SERVING others, society expects that women will land a job and then handle homemaking and childrearing on the side. When culture shifted and this became the norm in society, homemaking began to become a lost art for wives.

    Also, a cake-baking class sounds super fun. I’m kinda praying now that God would show me a friend to do this with!


  16. arjoomand says:

    loved this article !….. it takes every woman a while to realise that homemaking needs continuous learning and growing just like any other profession….

  17. Sarah L says:

    I love, love, LOVE this article. I became a SAHM pretty suddenly (after quitting my teaching job, ironically) and had to learn the ins and outs of homemaking pretty quickly. I based my new blog on the fact that being a SAHM is my job and I try to make the most of it everyday. Thank you for this article and the reassurance that what we do is one of the most important jobs out there!

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